Year *

Objectives (the objective name)

Associated activities

2007

1

To gather evidence for finalize of selection criteria on clinical, histopathological, immunohistochemical and morphometrical basis.

1.1. Thoroughly studies on tumor and nontumoral angiogenesis and lymphangiogenesis process.

1.2. Literature studies regarding oral squamous cell carcinoma angiogenesis and lymphangiogenesis

1.3. Literature studies regarding blood and lymphatic endothelial cell markers, pro- and antiangiogenic and pro- and anti-lymph angiogenic factors implicated in oral carcinogenesis

1.4 Workshop of the research team for homogenization of knowledge and to establish of the future research phases

1.5 Establishing of the research criteria and the statistics selection methods

1.6. Made of a site for results dissemination

2008

1

The clinical and histopathological preliminary studies in OSCCs

1.1. Retrospective studies for selection of the clinical cases and the registration of its in the data base

1.2 Prospective studies for identification of new clinical cases and the registration of its in the data base

1.3. Sample prelevation, histopathological processing and sample storage

1.4. Clinical and histopathological statistics process of the acquired data

1.5. Identification of the concerning cases which will be process by immunohistochemistry and morphometry

1.6. Workshop of the research team for homogenization of knowledge and to establish of the future research phases

2

The preliminary immunohistochemical and morphometrical studies of neoangiogenesis in OSCC

2.1. Immunohistochemical identification of the main blood new vessels from oral squamous cell carcinoma (OSCC)

2.2. Establishing of the new blood vessels maturity from OSCC

2.3. The evaluation of the intra- and peritumoral new vessel microdensity in OSCC

2.4. Evaluation of the implication degree of pro- and antiangiogenic factors in OSCC

2.5. Statistical process of the acquired data

3

The preliminary immunohistochemical and morphometrical studies of lymph angiogenesis in OSCC

3.1. Immunohistochemical identification of the lymph new vessels from OSCC

3.2. Quantification of the proliferation degree for lymph endothelial cells from OSCC

3.3. The evaluation of the intra- and peritumoral new lymph vessel microdensity from superficial and deep parts of OSCC

3.4. Evaluation of the implication degree of pro- and anti lymph angiogenic factors in OSCC

3.5. Statistical process of the acquired data

4

Acquiring of the partially research results and their disseminations

4.1. Workshop of the research team for elaboration of the future articles that will be publishes.

4.2. The site actualization.

2009

1

Continuing of the clinical and histopathological evaluations

1.1. Continuing of the clinical monitoring of the selected cases and their inclusion in data base

1.2. Continuing the sampling prelevation and their histopathological processing.

1.3. The histopathological analysis and the results statistical processing.

2

Continuing of the immunohistochemical and morphometric studies on neoangiogenesis in OSCC

2.1. Continuing of the immunohistochemical processing and of the main new blood vessels evaluation in OSCC

2.2 Evaluation of the of the new blood vessels maturity from OSCC

2.3. The evaluation of the intra- and peritumoral new vessel microdensity in OSCC

2.4. Evaluation of the implication degree of pro- and antiangiogenic factors in OSCC

2.5. Statistical process of the acquired data

3

Continuing of the immunohistochemical and morphometric studies on lymph angiogenesis in OSCC

3.1. Immunohistochemical evaluation of the new lymph vessels from OSCC

3.2. Continuing of the endothelial lymph cell proliferate degree evaluation in OSCC

3.3. The evaluation of the intra- and peritumoral new lymph vessel microdensity from superficial and deep parts of OSCC

3.4. Evaluation of the implication degree of pro- and anti lymph angiogenic factors in OSCC

3.5. Statistical process of the acquired data

4

Acquiring of the partially research results and their disseminations

4.1. Workshop of the research team for elaboration of the future articles that will be publishes

4.2. The site actualization

2010

1

The complex histopathological, immunohistochemical and morphometrical evaluation of the angiogenic and lymphangiogenic phenotype in OSCC

1.1. Continuing of the clinical monitoring of the selected cases

1.2. Comparative morphometrical analysis of blood and lymph microvessels density from inside and periphery, respective from superficial and deep parts of the OSCC

1.3. Comparative immunohistochemical analysis of the main pro angiogenic and pro lymph angiogenic factors and for their receptors at the endothelial blood and lymph vessel in OSCCC

1.4. Comparative immunohistochemical analysis of the main anti angiogenic and anti lymph angiogenic factors in OSCC

1.5. Statistical process of the acquired data

1.6. The angiogenic phenotype characterization in different variants of OSCC

1.7. The lymph angiogenic phenotype characterization in different variants of OSCC

1.8. Establishing of the most significant statistical correlations between morfoclinical parameters and blood and lymph microvessels density, the expression degree of pro-angiogenic and pro-lymph angiogenic factors and their receptors in OSCC

1.9. Establishing of the main function of antiangiogenic and anti-lymph angiogenic factors in OSCC

1.10. Quantification of the prognostic value of angiogenesis and lymphangiogenesis in OSCC

2

Acquiring of the final research results and their disseminations

2.1. Workshop of the research team for elaboration of the future articles that will be publishes

2.2. The site actualization

2.3. Programming and organize of a conference for dissemination of the final results

2.4. Identification of the following methods for the following researches